WP3: sample preparation methods and particle fractionaction in biological matrices

Main Goal:

To harmonise and standardise robust and efficient sample preparation methods for the measurement of nanoparticle stability and their biotransformation in liquid biological matrices, minimising matrix interferences.

 

 

Techniques:

1. Fractionation strategies to separate particles from proteins and other matrix components, including size exclusion chromatography (SEC), asymmetric flow field flow fractionaction (AF4) and analytical ultracentrifugation (AUC). Special attention will be paid to particle recovery rates (that should be ≥ 70 %) and repeatability/reproducibility of the methods applied to different formulations;
2. Bio-SAXS compared to the hyphenation of AF4 with SAXS

 

 

WP3 Leader:

Enrica Alasonati
LNE, France

Short bio: Enrica Alasonati is a permanent researcher at LNE since 2011. She obtained her PhD in Environmental Chemistry in 2009 at EPFL (Ecole Polytechnique Fédérale de Lausanne), Switzerland, working on the development of fractionation techniques for the characterization of environmental colloids. She is an expert in fractionation techniques and nanoparticles analysis, responsible for the speciation analysis activities in the department and she is vice-chairman of the G4F, French FFF group in the French Separative Science Association. She was responsible for several tasks/WP in several EMRP/EMPIR JRPs (ENV08 WFD, ENV51 MeTra, METVES II, PlasticTrace) and participates today in projects and workgroups devoted to the development of reliable methods for the characterisation of nanomedicines, nano-food additives and micro and nanoplastics in  complex environments, including biological, environment and food matrices.